| Gene | Artn |
| Chromosome | 4 |
| Deletion Start | 117,927,680 |
| Deletion End | 117,926,563 |
| Deletion Size | 1,118 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,927,468 | 117,927,488 | CGTGCCCTCAGGTAATTCCTC |
| TUP | 117,927,443 | 117,927,465 | CTGGGCTCCTGGGAGCAGCAAAT |
| TUR | 117,927,401 | 117,927,420 | GCTGGGTGGCAGAGTGTAAG |
| TDF | 117,926,656 | 117,926,675 | AGGCCGTCTCCTTCATGGAC |
| TDP | 117,926,633 | 117,926,654 | TGAACAGCACCTGGAGGACCGT |
| TDR | 117,926,563 | 117,926,585 | AGTGTGCAAAGGCTTGGAGATAG |
PCR Primers
| Primer | Sequence |
| SU | CTTTGTTTGCCCATCTGGAG |
| SD | CACAAGTGAACACGGGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12783A-A9 | VGB6 | C57BL/6NTac | |
| 12783A-D4 | VGB6 | C57BL/6NTac | |
| 12783A-F3 | VGB6 | C57BL/6NTac | |
