| Gene | Cdk5r1 |
| Chromosome | 11 |
| Deletion Start | 80,477,512 |
| Deletion End | 80,478,518 |
| Deletion Size | 1,007 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,477,606 | 80,477,625 | GCAAGAACGCCAAGGACAAG |
| TUP | 80,477,628 | 80,477,652 | CCTGAAGCGGCACTCCATCATCTCG |
| TUR | 80,477,660 | 80,477,679 | CGCCACGATCCTCTTCCAAG |
| TDF | 80,478,077 | 80,478,096 | GGCAGGACCAGGGTTTCATC |
| TDP | 80,478,098 | 80,478,120 | CACCGGCCAATGTGGTCTTCCTC |
| TDR | 80,478,179 | 80,478,199 | ACAGACAGGTCAGCAGGACAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGTTGGATTCCTGCTGC |
| SD | GATCATGGGACAGTGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12784B-A11 | VGB6 | C57BL/6NTac | |
| 12784B-B7 | VGB6 | C57BL/6NTac | |
| 12784B-D3 | VGB6 | C57BL/6NTac | |
| 12784B-D12 | VGB6 | C57BL/6NTac | |
| 12784B-F9 | VGB6 | C57BL/6NTac | |
