| Gene | Pou3f4 |
| Chromosome | X |
| Deletion Start | 110,814,468 |
| Deletion End | 110,815,612 |
| Deletion Size | 1,145 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,814,823 | 110,814,844 | CAAACTCGTCCATCACGTCCAG |
| TUP | 110,814,853 | 110,814,877 | CCCTCAATGTGTACTCGCAGCCAGG |
| TUR | 110,814,885 | 110,814,903 | GCTCCAGCATACCGCTCAC |
| TDF | 110,815,495 | 110,815,516 | CACACGAGGTTTATTCGCACAC |
| TDP | 110,815,528 | 110,815,550 | ACGCGTCCTGCCACGATCTCTGA |
| TDR | 110,815,551 | 110,815,570 | CACCTCCTTGCTTCCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGCGACCTGAAACTGAAG |
| SD | ACACATCTGTCTTCCCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12785A-C6 | VGB6 | C57BL/6NTac | |
| 12785A-D6 | VGB6 | C57BL/6NTac | |
