| Gene | Dusp2 |
| Chromosome | 2 |
| Deletion Start | 127,336,241 |
| Deletion End | 127,337,819 |
| Deletion Size | 1,579 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,336,733 | 127,336,755 | CCCGACCTCTTGGCTAATCATAC |
| TUP | 127,336,756 | 127,336,781 | CCTTTCTTGTCTTTCCCACAGGCGGT |
| TUR | 127,336,812 | 127,336,831 | GCAGGAGCTTCAGAGCACAG |
| TDF | 127,337,488 | 127,337,508 | GGGTTCTGTGGGAACTGTAGG |
| TDP | 127,337,514 | 127,337,541 | AGGTACAGGCACCTGATTCCTCTGTCCA |
| TDR | 127,337,551 | 127,337,571 | AAATGTGGAGCCGAGAGTCAG |
PCR Primers
| Primer | Sequence |
| SU | ACTTGTCACGGTCTGGTTCC |
| SD | ACACACACGTCACTTCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12786A-A8 | VGB6 | C57BL/6NTac | |
| 12786A-B7 | VGB6 | C57BL/6NTac | |
| 12786A-C3 | VGB6 | C57BL/6NTac | |
| 12786A-F10 | VGB6 | C57BL/6NTac | |
