| Gene | Sox18 |
| Chromosome | 2 |
| Deletion Start | 181,671,518 |
| Deletion End | 181,670,167 |
| Deletion Size | 1,352 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,671,248 | 181,671,265 | TCATGGTGTGGGCGAAGG |
| TUP | 181,671,224 | 181,671,244 | AGCGCAAGCGACTGGCGCAAC |
| TUR | 181,671,173 | 181,671,192 | GGCTCTCACCCAGCATCTTG |
| TDF | 181,670,430 | 181,670,448 | CCGTCTCTTGAGGGCACAG |
| TDP | 181,670,408 | 181,670,429 | AGCAACTGGAGCCTACCGCCGA |
| TDR | 181,670,376 | 181,670,397 | CAAATTCGGTGAGGTCCACATC |
PCR Primers
| Primer | Sequence |
| SU | TGAGACAGTGGGAGCAGATG |
| SD | ATGGAGGGCAGTGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12788B-C6 | VGB6 | C57BL/6NTac | |
| 12788B-H8 | VGB6 | C57BL/6NTac | |
| 12788B-H10 | VGB6 | C57BL/6NTac | |
| 12788B-F10 | VGB6 | C57BL/6NTac | |
