| Gene | Adam4 |
| Chromosome | 12 |
| Deletion Start | 81,421,842 |
| Deletion End | 81,419,553 |
| Deletion Size | 2,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,421,028 | 81,421,052 | TTTGGAATACGAAGAACCTGCATAC |
| TUP | 81,421,003 | 81,421,025 | TCCCCGGTGAAAATCCCCACCTT |
| TUR | 81,420,967 | 81,420,990 | CCATACACCAAAACCTGCCATAAC |
| TDF | 81,419,819 | 81,419,838 | GGCAACTGTCACTGTGTTCG |
| TDP | 81,419,792 | 81,419,818 | AGGATGGCAGCCACCACATTGTTTACA |
| TDR | 81,419,712 | 81,419,738 | CACTCACATGTATTTCAGCTTGATGTT |
PCR Primers
| Primer | Sequence |
| SU | TGGATGTAATTAAATGGTCAATGC |
| SD | AGAGCACATCCTCATAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12791A-A8 | VGB6 | C57BL/6NTac | |
| 12791A-B11 | VGB6 | C57BL/6NTac | |
| 12791A-E3 | VGB6 | C57BL/6NTac | |
| 12791A-G10 | VGB6 | C57BL/6NTac | |
