| Gene | P2ry1 |
| Chromosome | 3 |
| Deletion Start | 61,003,445 |
| Deletion End | 61,004,563 |
| Deletion Size | 1,119 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,003,562 | 61,003,581 | CAATGTGCCCTGACCAAGAC |
| TUP | 61,003,585 | 61,003,610 | TTTCCAGTTCTACTACCTGCCGGCTG |
| TUR | 61,003,645 | 61,003,664 | TCCAGATAGCCACGCTGTTG |
| TDF | 61,004,070 | 61,004,094 | ACCTGCGAAGTTATTTCATCTACAG |
| TDP | 61,004,097 | 61,004,119 | TGTGCACGACTGTGGCCATGTTC |
| TDR | 61,004,127 | 61,004,146 | GCCCAAGATCAGCACCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGAACAGCCGAACTACTTG |
| SD | TCACAGGTTCAAGTTCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12793C-A12 | VGB6 | C57BL/6NTac | |
| 12793C-F7 | VGB6 | C57BL/6NTac | |
| 12793C-H7 | VGB6 | C57BL/6NTac | |
