| Gene | Eif1 |
| Chromosome | 11 |
| Deletion Start | 100,320,133 |
| Deletion End | 100,321,415 |
| Deletion Size | 1,283 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,320,620 | 100,320,639 | TTCCCGGAGGGACAACAAAG |
| TUP | 100,320,642 | 100,320,670 | CACCTCCGGATTATTTCTTCACAGACCCC |
| TUR | 100,320,692 | 100,320,711 | CCAGCAGGAAGCAGGTCATC |
| TDF | 100,320,885 | 100,320,905 | ACGTCAAGGGAGTCCGTGTTG |
| TDP | 100,320,907 | 100,320,930 | TGGCATGGCCTCTTAGATGCTGCA |
| TDR | 100,320,996 | 100,321,017 | GGTCACCCTGTAGCTGAATTAC |
PCR Primers
| Primer | Sequence |
| SU | CGCTCAGACAACTACCATGC |
| SD | CAGATACCAGGGCCAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12794B-A12 | VGB6 | C57BL/6NTac | |
| 12794B-G3 | VGB6 | C57BL/6NTac | |
| 12794B-E11 | VGB6 | C57BL/6NTac | |
