| Gene | D10Wsu102e |
| Chromosome | 10 |
| Deletion Start | 83,360,262 |
| Deletion End | 83,365,981 |
| Deletion Size | 5,720 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,360,869 | 83,360,890 | CCAGGTGGTCCTTGTAGAGTTC |
| TUP | 83,360,892 | 83,360,914 | TTAGGAACCGCCCAGCCTGGCAG |
| TUR | 83,360,915 | 83,360,934 | TGCCTCTGCCTCTCCAAATG |
| TDF | 83,365,790 | 83,365,811 | CTCGGAGTCCGAAGAAGATGTC |
| TDP | 83,365,812 | 83,365,836 | TGCGTTCCCTCTGAAGTCACCATCG |
| TDR | 83,365,853 | 83,365,873 | CCTTTGCCACCTTCTGCATTC |
PCR Primers
| Primer | Sequence |
| SU | ACCACCTTCTTCATGCTTGC |
| SD | ACCGTCACACCTTAAATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12795B-A1 | VGB6 | C57BL/6NTac | |
| 12795B-A11 | VGB6 | C57BL/6NTac | |
| 12795B-B7 | VGB6 | C57BL/6NTac | |
| 12795B-C4 | VGB6 | C57BL/6NTac | |
| 12795B-D8 | VGB6 | C57BL/6NTac | |
| 12795B-F4 | VGB6 | C57BL/6NTac | |
