12797   Ly6e     Status: ES cell colonies screened / QC positives

GeneLy6e
Chromosome15
Deletion Start74,957,811
Deletion End74,958,778
Deletion Size968
Genome Build38
MGI IDMGI:106651
NCBI Gene ID17069
BAC ID285C11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,958,04274,958,061CTGGCCCAGCTACACCTTTG
TUP74,958,06374,958,090CACCTCGTGTTGACTTTAGCCACTCCAG
TUR74,958,10574,958,124GCCAGCCAATGACAACTGAC
TDF74,958,33574,958,357CATGCCAGGAGAAAGACCATTAC
TDP74,958,35874,958,382TGTATCACGTTATCTGCCGCTGCGG
TDR74,958,38474,958,407GAAACAGGCAGCTACTCACCAAAG

PCR Primers

PrimerSequence
SUGAGGCTCTCAGATGAATGGC
SDTTCAGGTCTTCTTTGCCCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR83 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12797A-D2VGB6C57BL/6NTac
12797A-D8VGB6C57BL/6NTac
12797A-H2VGB6C57BL/6NTac
12797A-H3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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