| Gene | Neurog1 |
| Chromosome | 13 |
| Deletion Start | 56,251,929 |
| Deletion End | 56,251,102 |
| Deletion Size | 828 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,251,641 | 56,251,660 | GAGGAGTCGTCGCGTCAAAG |
| TUP | 56,251,620 | 56,251,640 | CCAACGATCGCGAGCGCAACC |
| TUR | 56,251,523 | 56,251,543 | AAGCGCAGCGTCTCAATCTTG |
| TDF | 56,251,239 | 56,251,256 | TTCCTGGCCTGCCCAAAG |
| TDP | 56,251,218 | 56,251,237 | CCTGCTCCACACGACGCCCT |
| TDR | 56,251,195 | 56,251,217 | GGCCTAGTGGTATGGGATGAAAC |
PCR Primers
| Primer | Sequence |
| SU | AAATTATGCAAATAGCAGGCG |
| SD | ATCCATAATGCATGAAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12800A-A10 | VGB6 | C57BL/6NTac | |
| 12800A-C3 | VGB6 | C57BL/6NTac | |
| 12800A-D8 | VGB6 | C57BL/6NTac | |
