| Gene | Alppl2 |
| Chromosome | 1 |
| Deletion Start | 87,089,760 |
| Deletion End | 87,087,020 |
| Deletion Size | 2,741 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,089,520 | 87,089,541 | GCCCATTCAGACATCAGCTAAG |
| TUP | 87,089,489 | 87,089,517 | CCTCGTTATCCTCATGGGAGATGGTGAGA |
| TUR | 87,089,453 | 87,089,475 | GGTGTAGGAGAGTCCCAGGATAG |
| TDF | 87,087,808 | 87,087,831 | GCCACCATGAAACTGTAGCCTATC |
| TDP | 87,087,765 | 87,087,791 | TGTCATGTTCGACTCAGCTGTTGACAA |
| TDR | 87,087,707 | 87,087,727 | AGAGTGGTCGGCAGTGACAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCCTCAGCCACAGATTCAG |
| SD | TTTATGGCAGCCAGACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12802A-D6 | VGB6 | C57BL/6NTac | |
| 12802A-E9 | VGB6 | C57BL/6NTac | |
