| Gene | Mrpl49 |
| Chromosome | 19 |
| Deletion Start | 6,057,733 |
| Deletion End | 6,054,791 |
| Deletion Size | 2,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,057,482 | 6,057,501 | GACTGCGCTTGGAGAGTCTG |
| TUP | 6,057,457 | 6,057,479 | TTTGGGAATGTGGCTGCCGCATC |
| TUR | 6,057,434 | 6,057,455 | TCTCGGTCTTACCCAACTCTTG |
| TDF | 6,055,293 | 6,055,312 | TGAGACCAGCAAACCCTGAC |
| TDP | 6,055,268 | 6,055,292 | AGAGCCCTTTGCAGCTTCGGTGAGG |
| TDR | 6,055,239 | 6,055,259 | GCCTCATCCACCTCAACCAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAACCGCCCTTGTACTTTG |
| SD | AGTTAATATCTGCTTTTGATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12803B-C1 | VGB6 | C57BL/6NTac | |
| 12803B-E6 | VGB6 | C57BL/6NTac | |
| 12803B-E10 | VGB6 | C57BL/6NTac | |
| 12803B-G5 | VGB6 | C57BL/6NTac | |
