| Gene | Tmsb10 |
| Chromosome | 6 |
| Deletion Start | 72,958,009 |
| Deletion End | 72,957,517 |
| Deletion Size | 493 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,957,930 | 72,957,950 | CGAGACGCAGGAGAAGAACAC |
| TUP | 72,957,902 | 72,957,927 | TGCCGACCAAAGAGAGTAAGTGTGCC |
| TUR | 72,957,863 | 72,957,882 | CACAGCAGGGAAGGGAGGAG |
| TDF | 72,957,669 | 72,957,691 | TGTAAGGTAGCTCCAATGACCAC |
| TDP | 72,957,646 | 72,957,667 | TCCCTCCCTTCGCAGCCATTGA |
| TDR | 72,957,621 | 72,957,644 | GGAGATTTCACTCCTCTTTTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAATCCACGGTAAGGCAC |
| SD | GACATGGATCGCATTAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12804A-D2 | VGB6 | C57BL/6NTac | |
| 12804A-E3 | VGB6 | C57BL/6NTac | |
| 12804A-F5 | VGB6 | C57BL/6NTac | |
