| Gene | Mnx1 |
| Chromosome | 5 |
| Deletion Start | 29,478,272 |
| Deletion End | 29,473,846 |
| Deletion Size | 4,427 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,477,587 | 29,477,606 | CCCAAGATGCCGGACTTCAG |
| TUP | 29,477,565 | 29,477,586 | CTGTGAGTACCTGCGCCGCACC |
| TUR | 29,477,542 | 29,477,560 | AGGATGCGCGTGAGAACTG |
| TDF | 29,473,989 | 29,474,012 | GAGGACGAGGATGATGAAGAAGAG |
| TDP | 29,473,962 | 29,473,987 | ACAACTTCCCGTACAGCAATGGTGCC |
| TDR | 29,473,928 | 29,473,948 | CAGATGAGCAGTCGGATGAGG |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTAATTGGACCGTGAC |
| SD | TCTCCAAAGGAGGGTTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12805A-F8 | VGB6 | C57BL/6NTac | |
| 12805A-E12 | VGB6 | C57BL/6NTac | |
