| Gene | Kifc2 |
| Chromosome | 15 |
| Deletion Start | 76,660,670 |
| Deletion End | 76,667,506 |
| Deletion Size | 6,837 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,661,376 | 76,661,395 | CTGGGCAAGGTGAGCGATAC |
| TUP | 76,661,399 | 76,661,425 | CGCCAGCAATTATTCCTTTCCCTAGCC |
| TUR | 76,661,437 | 76,661,458 | AAGCAGAGGTGTTGGGAGTGAG |
| TDF | 76,666,676 | 76,666,696 | GGAGAAGCAACCGAGCTACAG |
| TDP | 76,666,698 | 76,666,722 | AGCCACTGTCATGAACCAGCACAGC |
| TDR | 76,666,741 | 76,666,762 | CTGCACGTAAAGTCAGCGTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGAGCTGAAGGCTCGGTTC |
| SD | CTCAAATGCCTGGGTAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12806A-D5 | VGB6 | C57BL/6NTac | |
| 12806A-C4 | VGB6 | C57BL/6NTac | |
| 12806A-B12 | VGB6 | C57BL/6NTac | |
| 12806A-A3 | VGB6 | C57BL/6NTac | |
