| Gene | Tuba4a |
| Chromosome | 1 |
| Deletion Start | 75,219,192 |
| Deletion End | 75,215,561 |
| Deletion Size | 3,632 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,218,410 | 75,218,429 | CCCAGGTACAGATGGCGTTC |
| TUP | 75,218,389 | 75,218,409 | CCCTGGCTTGTGCAGCAAGGC |
| TUR | 75,218,367 | 75,218,386 | TAGGAGGCCCTTGAGGAAAC |
| TDF | 75,216,000 | 75,216,019 | TGCTGTACCGTGGAGATGTG |
| TDP | 75,215,975 | 75,215,998 | TGCCCAAAGATGTGAATGCTGCCA |
| TDR | 75,215,949 | 75,215,967 | GCTGCGCTTCGTCTTGATG |
PCR Primers
| Primer | Sequence |
| SU | GGATTGCTTGAGCTTCATCC |
| SD | TTGAGTGAAGCAGCAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12811A-D5 | VGB6 | C57BL/6NTac | |
| 12811A-D10 | VGB6 | C57BL/6NTac | |
| 12811A-B2 | VGB6 | C57BL/6NTac | |
| 12811A-H11 | VGB6 | C57BL/6NTac | |
