| Gene | Endog |
| Chromosome | 2 |
| Deletion Start | 30,171,578 |
| Deletion End | 30,174,066 |
| Deletion Size | 2,489 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,172,359 | 30,172,380 | CAGCAGGGTCTTGATTGTGAAG |
| TUP | 30,172,382 | 30,172,403 | AGGTTCCTGGGACATCCACCCA |
| TUR | 30,172,407 | 30,172,428 | TGGTCTCCTGTCCATCTCCATC |
| TDF | 30,173,090 | 30,173,109 | CCACGTCTGACCTGCCTTAC |
| TDP | 30,173,111 | 30,173,133 | ACCTGTAACCCGTGGCTGCACGG |
| TDR | 30,173,166 | 30,173,187 | GCTCATTTGTCTGCCAGGAATC |
PCR Primers
| Primer | Sequence |
| SU | ACATACTTCCCAGGCCAAAC |
| SD | CACCCATCCTGGAGACTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12818A-E1 | VGB6 | C57BL/6NTac | |
| 12818A-F4 | VGB6 | C57BL/6NTac | |
| 12818A-E5 | VGB6 | C57BL/6NTac | |
