| Gene | Cldn5 |
| Chromosome | 16 |
| Deletion Start | 18,776,999 |
| Deletion End | 18,777,714 |
| Deletion Size | 716 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,777,271 | 18,777,292 | GGTGGCACTCTTTGTTACCTTG |
| TUP | 18,777,293 | 18,777,312 | ACCGGCGCTCAGTGCACCAC |
| TUR | 18,777,330 | 18,777,349 | TGCTACCCGTGCCTTAACTG |
| TDF | 18,777,416 | 18,777,436 | AACATCGTTGTCCGCGAGTTC |
| TDP | 18,777,437 | 18,777,459 | TATGATCCGACGGTGCCGGTGTC |
| TDR | 18,777,461 | 18,777,479 | CGCCCAGCTCGTACTTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGGTCATGCTGCTAACAGTG |
| SD | CTGCCCTTTCAGGTTAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12819A-A5 | VGB6 | C57BL/6NTac | |
| 12819A-C6 | VGB6 | C57BL/6NTac | |
| 12819A-E2 | VGB6 | C57BL/6NTac | |
| 12819A-D6 | VGB6 | C57BL/6NTac | |
