| Gene | Utf1 |
| Chromosome | 7 |
| Deletion Start | 139,943,877 |
| Deletion End | 139,945,010 |
| Deletion Size | 1,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,944,216 | 139,944,233 | CGCCGTCGCTACAAGTTC |
| TUP | 139,944,235 | 139,944,259 | TCAAGGACAAACTCCGAGACTCCCA |
| TUR | 139,944,287 | 139,944,305 | CATGAGTTGGCGGATCTGG |
| TDF | 139,944,820 | 139,944,838 | TTGGGCGACATCTCAACAG |
| TDP | 139,944,839 | 139,944,858 | TTCTGGGCCCTCTGCGCGAC |
| TDR | 139,944,902 | 139,944,922 | GAGAAACGGTTTGGTCGAAGG |
PCR Primers
| Primer | Sequence |
| SU | ACACGTTCTGTTTTGTGTAGC |
| SD | TCCACGTACCTTATCCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12820A-E1 | VGB6 | C57BL/6NTac | |
| 12820B-E10 | VGB6 | C57BL/6NTac | |
