| Gene | F2rl3 |
| Chromosome | 8 |
| Deletion Start | 72,761,906 |
| Deletion End | 72,763,384 |
| Deletion Size | 1,479 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,762,065 | 72,762,087 | GAGCAGGCTTACAGTCTTAGGTC |
| TUP | 72,762,088 | 72,762,114 | CCTAAACTCCATGCCTTTGGGACTGGG |
| TUR | 72,762,149 | 72,762,166 | TGTGGGTGCCTCAGTGTC |
| TDF | 72,762,686 | 72,762,707 | TGCAGTCAGCTTGGACAGATAC |
| TDP | 72,762,709 | 72,762,729 | TGGCCCTGGTGCATCCTTTGC |
| TDR | 72,762,760 | 72,762,781 | GCCACCAAACAGAGTCCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGGATGTTGTGGAGATTTGG |
| SD | ATCATCCTTTGGCCTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12821A-C4 | VGB6 | C57BL/6NTac | |
| 12821A-D2 | VGB6 | C57BL/6NTac | |
| 12821A-D11 | VGB6 | C57BL/6NTac | |
| 12821A-E4 | VGB6 | C57BL/6NTac | |
