| Gene | Dio3 |
| Chromosome | 12 |
| Deletion Start | 110,279,313 |
| Deletion End | 110,280,159 |
| Deletion Size | 847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,279,428 | 110,279,447 | GCATCCGCAAGCATTTCCTG |
| TUP | 110,279,448 | 110,279,467 | CGCCGTCGCCATCCTGACCA |
| TUR | 110,279,489 | 110,279,510 | CATCTCCTCGCCTTCACTGTTG |
| TDF | 110,279,949 | 110,279,969 | GCTCTGGTCCTGGACACTATG |
| TDP | 110,279,976 | 110,279,999 | TCTAGCAGTTCCGCATATGGTGCC |
| TDR | 110,280,067 | 110,280,088 | CGCAACTCAGACACCTGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ACTGACTGCTTGGCTCTGAG |
| SD | AAGTCCGAGCTGTGAAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12823A-B8 | VGB6 | C57BL/6NTac | |
| 12823A-D10 | VGB6 | C57BL/6NTac | |
| 12823A-D11 | VGB6 | C57BL/6NTac | |
| 12823A-H4 | VGB6 | C57BL/6NTac | |
