| Gene | S1pr4 |
| Chromosome | 10 |
| Deletion Start | 81,499,635 |
| Deletion End | 81,498,391 |
| Deletion Size | 1,245 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,499,324 | 81,499,342 | CCTGGCCTACGTGGTCAAC |
| TUP | 81,499,298 | 81,499,322 | TGCTGCTGTCAGGGACTCGTACCTT |
| TUR | 81,499,211 | 81,499,231 | GCCGTGAAGAGCAGACTGAAG |
| TDF | 81,498,494 | 81,498,513 | CCGCTGTCCAGCATTTCCAG |
| TDP | 81,498,470 | 81,498,491 | TCCGCAGCACCTAGAGCTTGGA |
| TDR | 81,498,395 | 81,498,415 | CCATCAGGCAAGGTTCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | ATCTTGGATGCCAGGCTATG |
| SD | GGCTGCTTGTCCCAATGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12827A-A10 | VGB6 | C57BL/6NTac | |
| 12827A-D2 | VGB6 | C57BL/6NTac | |
| 12827A-H3 | VGB6 | C57BL/6NTac | |
| 12827A-E5 | VGB6 | C57BL/6NTac | |
