| Gene | Rce1 |
| Chromosome | 19 |
| Deletion Start | 4,625,614 |
| Deletion End | 4,622,984 |
| Deletion Size | 2,631 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,624,869 | 4,624,888 | TGGCTGGGAAACCCTAGAAC |
| TUP | 4,624,838 | 4,624,863 | AAACCTGAGGTCTGGTGAGGCTGACC |
| TUR | 4,624,810 | 4,624,828 | CAATGACCTTGCCCACACC |
| TDF | 4,623,564 | 4,623,584 | ACTCGCAGCCTCCATCTCCAG |
| TDP | 4,623,538 | 4,623,563 | CGTTCCAGTTCTCCTACACCGCTGTC |
| TDR | 4,623,496 | 4,623,516 | CCAACCTGTGCGGATGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTCACTGCTAGAGGATCG |
| SD | AACCCTGGGAAGTTGAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12828A-A3 | VGB6 | C57BL/6NTac | |
| 12828A-A7 | VGB6 | C57BL/6NTac | |
| 12828A-B8 | VGB6 | C57BL/6NTac | |
| 12828A-G10 | VGB6 | C57BL/6NTac | |
