| Gene | Cst9 |
| Chromosome | 2 |
| Deletion Start | 148,835,214 |
| Deletion End | 148,838,562 |
| Deletion Size | 3,349 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,835,341 | 148,835,362 | CAGTGGAATTTGCCGTGAACAC |
| TUP | 148,835,365 | 148,835,393 | TCAACCAGGAAAGTCAGGATGAATATGCC |
| TUR | 148,835,414 | 148,835,434 | ACCTTCTCCCTCCAGGAACTC |
| TDF | 148,837,678 | 148,837,701 | AATTTCTGCCATGTGGCTAGTTAC |
| TDP | 148,837,703 | 148,837,730 | TCTGCTCAGCTCTCATGTGTCTATCCTC |
| TDR | 148,837,752 | 148,837,773 | TCTGGGATTGAGCCAAGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTCCTCAGATGCTCTCCC |
| SD | AGAGTGTCAGAGACCTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12829A-H8 | VGB6 | C57BL/6NTac | |
| 12829A-B11 | VGB6 | C57BL/6NTac | |
| 12829A-D8 | VGB6 | C57BL/6NTac | |
| 12829A-G6 | VGB6 | C57BL/6NTac | |
| 12829A-A8 | VGB6 | C57BL/6NTac | |
| 12829A-H5 | VGB6 | C57BL/6NTac | |
