| Gene | Pbx2 |
| Chromosome | 17 |
| Deletion Start | 34,592,557 |
| Deletion End | 34,595,975 |
| Deletion Size | 3,419 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,593,374 | 34,593,393 | CCCACTGCCTGCATCTTCTG |
| TUP | 34,593,394 | 34,593,416 | CTTCCTGTGACCCTGCCGCTTCC |
| TUR | 34,593,436 | 34,593,456 | GGAGTCCGAGGGATAGTTGAG |
| TDF | 34,595,792 | 34,595,814 | TTCCCATTCTGACTCTCATTCTC |
| TDP | 34,595,817 | 34,595,839 | TTACCAGGCCAATGGTGGCTGGC |
| TDR | 34,595,874 | 34,595,893 | CACTTCCTGGTCCCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTCGGAGAACATTCCAGAG |
| SD | GCCTCTGTCCAAGAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12831A-F9 | VGB6 | C57BL/6NTac | |
| 12831A-G9 | VGB6 | C57BL/6NTac | |
| 12831A-A12 | VGB6 | C57BL/6NTac | |
| 12831A-A1 | VGB6 | C57BL/6NTac | |
