| Gene | Hey1 |
| Chromosome | 3 |
| Deletion Start | 8,666,867 |
| Deletion End | 8,664,432 |
| Deletion Size | 2,436 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,666,256 | 8,666,280 | GAGAAGCAGGTAATGGAAGTATGAG |
| TUP | 8,666,229 | 8,666,253 | CGCTGAGTGTAGCCATCGGCACCTT |
| TUR | 8,666,179 | 8,666,200 | CCAACGACTTGGCACAAATGAG |
| TDF | 8,664,642 | 8,664,660 | TCGGCCTCCAAACTGTCTC |
| TDP | 8,664,620 | 8,664,641 | CACCGCTGCTCTCCTCTGTGGC |
| TDR | 8,664,563 | 8,664,582 | GTCGAAGGGCTCAGTAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCAATCTGCGCAGCGAG |
| SD | AACACCTCGGTCCATCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12832A-B7 | VGB6 | C57BL/6NTac | |
| 12832A-H5 | VGB6 | C57BL/6NTac | |
| 12832B-B3 | VGB6 | C57BL/6NTac | |
| 12832B-B4 | VGB6 | C57BL/6NTac | |
| 12832B-B10 | VGB6 | C57BL/6NTac | |
| 12832B-D11 | VGB6 | C57BL/6NTac | |
| 12832B-F6 | VGB6 | C57BL/6NTac | |
| 12832B-H6 | VGB6 | C57BL/6NTac | |
