| Gene | Taf10 |
| Chromosome | 7 |
| Deletion Start | 105,744,315 |
| Deletion End | 105,742,911 |
| Deletion Size | 1,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,743,807 | 105,743,826 | CGCTTCCCACCTGAACAAGG |
| TUP | 105,743,783 | 105,743,805 | CACCTGGCTCGCTGTCAATGCCT |
| TUR | 105,743,728 | 105,743,752 | CAAAAGAGATGGCTTCATAGCAGAG |
| TDF | 105,743,226 | 105,743,243 | TCCCGGAGCAAGAGCAAG |
| TDP | 105,743,199 | 105,743,222 | TGAGAGGCTCACCTTCCACTGCAG |
| TDR | 105,743,130 | 105,743,148 | TGAGCAGGCCCAATACCTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGACCGTCAGGAGACTGG |
| SD | GAATGAAGACCCTGCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12833B-F4 | VGB6 | C57BL/6NTac | |
| 12833B-F5 | VGB6 | C57BL/6NTac | |
| 12833B-B12 | VGB6 | C57BL/6NTac | |
