| Gene | Tssk1 |
| Chromosome | 16 |
| Deletion Start | 17,894,356 |
| Deletion End | 17,895,544 |
| Deletion Size | 1,189 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,894,708 | 17,894,727 | AGCTCTCCTCGGCCATCAAG |
| TUP | 17,894,731 | 17,894,752 | TGCCACGACCTGGATGTCGTCC |
| TUR | 17,894,802 | 17,894,823 | GAAGCCGAAGTCAGACAGTTTG |
| TDF | 17,895,352 | 17,895,370 | CAGGTGGTCAGGCAGTCAG |
| TDP | 17,895,373 | 17,895,392 | AACGTGGGCCTCAGCAGCGA |
| TDR | 17,895,396 | 17,895,416 | CCTTCCTCTGTGTCCCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CATGAAGCCATTCTGTTCCC |
| SD | CCTCTTTGCAGTTCTCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12837A-A4 | VGB6 | C57BL/6NTac | |
| 12837A-B1 | VGB6 | C57BL/6NTac | |
| 12837A-D6 | VGB6 | C57BL/6NTac | |
| 12837A-H6 | VGB6 | C57BL/6NTac | |
