| Gene | Acot1 |
| Chromosome | 12 |
| Deletion Start | 84,009,513 |
| Deletion End | 84,017,386 |
| Deletion Size | 7,874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,010,171 | 84,010,192 | GACAGGATTGGACTGGAAGGAG |
| TUP | 84,010,195 | 84,010,222 | TGTTATGGTGATGCTGTTTGCCTGAAGC |
| TUR | 84,010,225 | 84,010,246 | CTTAGCAGCGGAATTGAAGGAC |
| TDF | 84,016,921 | 84,016,945 | AAGGATGAGACTATACCCCCTGTGA |
| TDP | 84,016,959 | 84,016,984 | TCAGGTCAAAATGACCAAGGATGGCC |
| TDR | 84,016,987 | 84,017,011 | TTTGCAAAGCATCTACAACATCCTT |
PCR Primers
| Primer | Sequence |
| SU | GTCAAAGACTGCCGGCTAAG |
| SD | ACTTCGGGAGGGAAGATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12838A-B5 | VGB6 | C57BL/6NTac | |
| 12838A-C4 | VGB6 | C57BL/6NTac | |
| 12838A-F9 | VGB6 | C57BL/6NTac | |
| 12838A-G1 | VGB6 | C57BL/6NTac | |
| 12838A-B2 | VGB6 | C57BL/6NTac | |
| 12838A-F11 | VGB6 | C57BL/6NTac | |
