12839   Deb1     Status: ES cell colonies screened / QC positives

GeneDeb1
Chromosome9
Deletion Start121,710,406
Deletion End121,712,687
Deletion Size2,282
Genome Build38
MGI IDMGI:1349474
NCBI Gene ID26901
BAC ID289O24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,710,617121,710,636TTCTTCCTCGCCCGTCTTTC
TUP121,710,637121,710,663AGCTCCTGGAGGAGAATGACCAGCTGA
TUR121,710,684121,710,702TCGCTCGGCCCTTATTCTG
TDF121,712,014121,712,032AGGCAGGCCAAAGGTGCTG
TDP121,712,037121,712,059TCCTCAGGCATACCGAGGAGGCC
TDR121,712,077121,712,095CCTGTGGCTTTCCCTTCAC

PCR Primers

PrimerSequence
SUACTGGAAGGAGCCTCTCAG
SDTGACATGGGACACTGAGGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR86 bp++-
TDFTDR82 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12839A-A11VGB6C57BL/6NTac
12839A-D5VGB6C57BL/6NTac
12839A-E6VGB6C57BL/6NTac
12839A-D10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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