| Gene | Tlx2 |
| Chromosome | 6 |
| Deletion Start | 83,070,076 |
| Deletion End | 83,068,707 |
| Deletion Size | 1,370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,069,938 | 83,069,957 | CCAGAGCCACGGAGAAAGTG |
| TUP | 83,069,909 | 83,069,931 | TTTCCAGTGGATTCCACGGAGCC |
| TUR | 83,069,856 | 83,069,876 | ACTCCCGAGCCTCTAGGCAAG |
| TDF | 83,068,843 | 83,068,861 | CGCTTTGCCTGCACAACTC |
| TDP | 83,068,818 | 83,068,841 | TCGCTCTTCGCGCTGCAGAATCTG |
| TDR | 83,068,779 | 83,068,797 | GGACACCGAAGCCACCTTG |
PCR Primers
| Primer | Sequence |
| SU | CAACTTAGCGTCCACCTGC |
| SD | GGCAGGAGACAACTGGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12840A-A6 | VGB6 | C57BL/6NTac | |
| 12840A-B1 | VGB6 | C57BL/6NTac | |
| 12840A-B11 | VGB6 | C57BL/6NTac | |
| 12840A-C11 | VGB6 | C57BL/6NTac | |
