| Gene | Tlx3 |
| Chromosome | 11 |
| Deletion Start | 33,203,456 |
| Deletion End | 33,201,263 |
| Deletion Size | 2,194 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,202,747 | 33,202,766 | AGCGAAATAGCGGAGCACTG |
| TUP | 33,202,715 | 33,202,733 | TCCACGTGTGGCCCGAACG |
| TUR | 33,202,693 | 33,202,711 | CCTTGAAGCCCAGCCCTAC |
| TDF | 33,201,403 | 33,201,422 | CACGACGCCTTCCAGAAGAG |
| TDP | 33,201,377 | 33,201,400 | TCAACGATTCCATCCAGCCCGACC |
| TDR | 33,201,344 | 33,201,363 | GAGCAAAGAGCGACGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GATGTCACCTTGGAACAGTC |
| SD | CGTGCACCCAGCCTAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12841A-D12 | VGB6 | C57BL/6NTac | |
| 12841A-D9 | VGB6 | C57BL/6NTac | |
| 12841A-C7 | VGB6 | C57BL/6NTac | |
| 12841A-A5 | VGB6 | C57BL/6NTac | |
