| Gene | Itgb1bp2 |
| Chromosome | X |
| Deletion Start | 101,449,176 |
| Deletion End | 101,453,457 |
| Deletion Size | 4,282 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,449,424 | 101,449,446 | ACCTTTCTGCCTTGATGTTTGTG |
| TUP | 101,449,453 | 101,449,482 | ATCTACAGATTCCTGTCGCTATCACCCTGG |
| TUR | 101,449,511 | 101,449,528 | GCATCGCCCATTCCTCAC |
| TDF | 101,452,515 | 101,452,538 | TGTAGTAGTGCTGACGGTCTATGG |
| TDP | 101,452,544 | 101,452,568 | TTCCGCTTCCTGCATTCAACTGGGT |
| TDR | 101,452,579 | 101,452,601 | GGAGTGTTCCTCACCTCAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GTACCCATACCCACACAGGG |
| SD | CTCTGTAGAGAAATGAAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12844A-E3 | VGB6 | C57BL/6NTac | |
| 12844A-F6 | VGB6 | C57BL/6NTac | |
| 12844A-H1 | VGB6 | C57BL/6NTac | |
| 12844A-D3 | VGB6 | C57BL/6NTac | |
