| Gene | Msgn1 |
| Chromosome | 12 |
| Deletion Start | 11,208,945 |
| Deletion End | 11,208,382 |
| Deletion Size | 564 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,208,781 | 11,208,801 | GCTCCTTCTCTGGAGTCCTAC |
| TUP | 11,208,760 | 11,208,780 | TCTGAGGTCGCACTGCCCTGC |
| TUR | 11,208,727 | 11,208,745 | ATCGCTGCCTCCTGTGCTG |
| TDF | 11,208,502 | 11,208,523 | GAATTACCTGCCGCCTGTCTAC |
| TDP | 11,208,479 | 11,208,501 | AGCCAGAGAGGCCAACCGCTCAC |
| TDR | 11,208,430 | 11,208,451 | TGTGAGTTCCCCGATGTACTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGTTCCACAGGACAATGC |
| SD | CTCCTTGCTTGACACAATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12849A-A3 | VGB6 | C57BL/6NTac | |
| 12849A-E8 | VGB6 | C57BL/6NTac | |
| 12849A-F2 | VGB6 | C57BL/6NTac | |
| 12849A-F10 | VGB6 | C57BL/6NTac | |
| 12849A-G10 | VGB6 | C57BL/6NTac | |
