| Gene | Pf4 |
| Chromosome | 5 |
| Deletion Start | 90,772,585 |
| Deletion End | 90,773,320 |
| Deletion Size | 736 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,772,805 | 90,772,825 | GATGCTGTAACCAAGCCTGTG |
| TUP | 90,772,829 | 90,772,853 | AGGTCCAGAGGTACCACACCGGCAG |
| TUR | 90,772,862 | 90,772,885 | GGGAGTAGAAGAGATAGCGATGAC |
| TDF | 90,773,074 | 90,773,095 | TCCTCTCCTCTGCTACCTGTTC |
| TDP | 90,773,098 | 90,773,123 | TCCCATCTCTTCCCTTCTTCCAACCA |
| TDR | 90,773,168 | 90,773,189 | CATTCTTCAGGGTGGCTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | ATTGGAGATGTGGATCCTGC |
| SD | GGCAACTCACTATGTTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12851A-A4 | VGB6 | C57BL/6NTac | |
| 12851A-B4 | VGB6 | C57BL/6NTac | |
| 12851A-C10 | VGB6 | C57BL/6NTac | |
| 12851A-D7 | VGB6 | C57BL/6NTac | |
| 12851A-C5 | VGB6 | C57BL/6NTac | |
