| Gene | Sf3b5 |
| Chromosome | 10 |
| Deletion Start | 13,008,626 |
| Deletion End | 13,008,984 |
| Deletion Size | 359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,008,703 | 13,008,721 | CAAGTGGGAATGGCTGGTG |
| TUP | 13,008,723 | 13,008,746 | ACCAGCACCGCGACTCCTACTGCT |
| TUR | 13,008,784 | 13,008,802 | GCTCTCGTTCTCGGCAATG |
| TDF | 13,008,863 | 13,008,882 | GACAAGCCCGAGGAGAACTG |
| TDP | 13,008,883 | 13,008,905 | AGGTGAGCGCTGCCCAGTCTTCC |
| TDR | 13,008,947 | 13,008,966 | GACACAGCCAACACGGAATG |
PCR Primers
| Primer | Sequence |
| SU | AGAACGTGATCACCCGAGAG |
| SD | CGACTTAAGGACTTGCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12854A-A10 | VGB6 | C57BL/6NTac | |
| 12854A-E7 | VGB6 | C57BL/6NTac | |
| 12854A-G3 | VGB6 | C57BL/6NTac | |
| 12854A-H3 | VGB6 | C57BL/6NTac | |
