| Gene | Nkapl |
| Chromosome | 13 |
| Deletion Start | 21,468,438 |
| Deletion End | 21,467,248 |
| Deletion Size | 1,191 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,468,141 | 21,468,165 | GGATACCGTTACCACCACAACTATG |
| TUP | 21,468,113 | 21,468,136 | AGATCCTTCGTGGGCTGAGGACCA |
| TUR | 21,468,073 | 21,468,094 | CAGCCTCCTTTGCCTATAACTC |
| TDF | 21,467,499 | 21,467,519 | GGAAGCGTATCCCACGAAGAG |
| TDP | 21,467,464 | 21,467,491 | TGGGTTGACAAGTGAAGAGATTGCCTCA |
| TDR | 21,467,422 | 21,467,440 | GCGATGCCTGCTACCACTC |
PCR Primers
| Primer | Sequence |
| SU | AGCAATACAGACAGACAGCAGG |
| SD | GTTCAGCTTCTTGGGCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12856A-B1 | VGB6 | C57BL/6NTac | |
| 12856A-D12 | VGB6 | C57BL/6NTac | |
