| Gene | Zfp830 |
| Chromosome | 11 |
| Deletion Start | 82,764,375 |
| Deletion End | 82,765,517 |
| Deletion Size | 1,143 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,764,861 | 82,764,883 | GGTGGAGAAGAAAGAAGGGACAG |
| TUP | 82,764,891 | 82,764,914 | CATCTTCCGGATGCACAGGGCAAA |
| TUR | 82,764,915 | 82,764,933 | GAGAGGCAAGCGAGTGTTC |
| TDF | 82,765,068 | 82,765,089 | ACCGCCGAAGCATTACCAGAAG |
| TDP | 82,765,094 | 82,765,118 | CTTCGACGACCCTGAGGTAGATGCA |
| TDR | 82,765,151 | 82,765,170 | CGTCCCACTCTTTGTCCATC |
PCR Primers
| Primer | Sequence |
| SU | AACCGGGTGTCTAAATGTCG |
| SD | AAACAACCTTCCCAAAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12860A-C8 | VGB6 | C57BL/6NTac | |
| 12860A-H1 | VGB6 | C57BL/6NTac | |
| 12860A-G2 | VGB6 | C57BL/6NTac | |
