| Gene | 4921517D21Rik |
| Chromosome | 9 |
| Deletion Start | 107,928,483 |
| Deletion End | 107,932,181 |
| Deletion Size | 3,699 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,929,087 | 107,929,109 | TCCAAGATCAGAGGCAAGACAAG |
| TUP | 107,929,113 | 107,929,136 | CCATGTCTGGCTCAGTCTCCCACC |
| TUR | 107,929,150 | 107,929,171 | AGAGGCAGTGACGGTGAAGAAG |
| TDF | 107,931,554 | 107,931,575 | TGCTGACCTCACTGATTACCTG |
| TDP | 107,931,578 | 107,931,601 | TCAGCTACTCTTGTCTGGTGGCCA |
| TDR | 107,931,611 | 107,931,632 | CTGTCTGATGATCCCTCCCTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGCAGGGAACACAGGAGAG |
| SD | AATTACCATGGCAACAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12866A-E6 | VGB6 | C57BL/6NTac | |
| 12866A-G9 | VGB6 | C57BL/6NTac | |
| 12866A-H8 | VGB6 | C57BL/6NTac | |
