| Gene | Tex13a |
| Chromosome | X |
| Deletion Start | 138,209,424 |
| Deletion End | 138,208,171 |
| Deletion Size | 1,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,209,100 | 138,209,119 | TGCACTCTCCTTGTCACCAG |
| TUP | 138,209,074 | 138,209,096 | TGAAGCAGCTCATCCACCAGCAA |
| TUR | 138,209,022 | 138,209,043 | GTTTGGCCTGAGCCATTTGAAG |
| TDF | 138,208,498 | 138,208,520 | TGTCCTCTGAGCCCTTTATTGTC |
| TDP | 138,208,466 | 138,208,493 | TCCCAGCCTCATTCACATACTCCTATGA |
| TDR | 138,208,422 | 138,208,441 | TGGTCGGTAGTTGGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | CTTTCTCCCTTGAAGCATGG |
| SD | GTCCACCAGGCACTAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12868A-E1 | VGB6 | C57BL/6NTac | |
| 12868A-B11 | VGB6 | C57BL/6NTac | |
