| Gene | Fam122a |
| Chromosome | 19 |
| Deletion Start | 24,477,353 |
| Deletion End | 24,476,414 |
| Deletion Size | 940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,477,033 | 24,477,051 | CGAGGTGCAGACCGCAATG |
| TUP | 24,477,002 | 24,477,027 | AAGCCACTCCTGGGAGGAAAGTTTCA |
| TUR | 24,476,980 | 24,476,999 | TCCACGTCGTTGTCACTCAG |
| TDF | 24,476,818 | 24,476,836 | GCCCAACCACCCGATTTAC |
| TDP | 24,476,794 | 24,476,817 | CACCCGGAGAAGCCAGAGTCCCAT |
| TDR | 24,476,754 | 24,476,776 | TCTTTTCAATGGTCCAAGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCTGAGCTCACACTTGC |
| SD | TTAGTGTGCCATGAACTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12869A-A4 | VGB6 | C57BL/6NTac | |
| 12869A-A6 | VGB6 | C57BL/6NTac | |
| 12869A-C7 | VGB6 | C57BL/6NTac | |
| 12869A-G6 | VGB6 | C57BL/6NTac | |
