| Gene | Tigd2 |
| Chromosome | 6 |
| Deletion Start | 59,210,153 |
| Deletion End | 59,211,770 |
| Deletion Size | 1,618 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,210,342 | 59,210,365 | GTGTCCAAGCGTAAATCCATGAAG |
| TUP | 59,210,366 | 59,210,392 | TCGTCAACGTATGAGGAGCTTGACAGA |
| TUR | 59,210,442 | 59,210,462 | GCTTTGCACAGATCGTTCCAG |
| TDF | 59,211,437 | 59,211,459 | CAGTGGTTTGACTCTCGGAGTAG |
| TDP | 59,211,460 | 59,211,486 | TGGCTCAAACTGTCAGGTGTTAGCTGA |
| TDR | 59,211,501 | 59,211,520 | AGTGACCTTCGCCCGGTCTG |
PCR Primers
| Primer | Sequence |
| SU | CTGCTCCGTGTTTCTGTCTG |
| SD | AGCAATGTTGTCTCCAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12870A-A6 | VGB6 | C57BL/6NTac | |
| 12870A-B3 | VGB6 | C57BL/6NTac | |
| 12870A-C8 | VGB6 | C57BL/6NTac | |
| 12870A-F6 | VGB6 | C57BL/6NTac | |
