| Gene | 1810011O10Rik |
| Chromosome | 8 |
| Deletion Start | 24,438,893 |
| Deletion End | 24,438,486 |
| Deletion Size | 408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,438,816 | 24,438,835 | CCATCCACGGCTACCACTTC |
| TUP | 24,438,791 | 24,438,813 | CACAGCCGCTCGCAAGAAAGCTG |
| TUR | 24,438,756 | 24,438,780 | GGACTCCTGGTCTATGTTCTCAAAG |
| TDF | 24,438,664 | 24,438,687 | CGACCAAGATTTGGAGGAGAAAAC |
| TDP | 24,438,642 | 24,438,663 | TCGAGCCCTCATGGCCCTGAAG |
| TDR | 24,438,569 | 24,438,591 | GCTGTGCTCAGTGTACCTTGATG |
PCR Primers
| Primer | Sequence |
| SU | TGGAAGGAAGAGCCAGTTTG |
| SD | TTCCTTCTCTTCCCTCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12873A-H5 | VGB6 | C57BL/6NTac | |
| 12873A-E6 | VGB6 | C57BL/6NTac | |
| 12873A-G6 | VGB6 | C57BL/6NTac | |
| 12873A-F8 | VGB6 | C57BL/6NTac | |
