| Gene | 2310009B15Rik |
| Chromosome | 1 |
| Deletion Start | 138,856,814 |
| Deletion End | 138,851,816 |
| Deletion Size | 4,999 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,856,779 | 138,856,796 | ACGCTGGCGAGGGTATCG |
| TUP | 138,856,759 | 138,856,778 | CTTGGGAGGCCACCGCCTCG |
| TUR | 138,856,714 | 138,856,732 | GTCGAACCGAGGGTTGAGT |
| TDF | 138,852,502 | 138,852,525 | ACAGTGTTCTGAAAGTCAGCCTTC |
| TDP | 138,852,473 | 138,852,496 | TGTGCAGTCTCCTGGCTATGCCAG |
| TDR | 138,852,425 | 138,852,447 | GGTGTGAGATTGACATGATGCTC |
PCR Primers
| Primer | Sequence |
| SU | TGCAGAATACGATCTAGCTTG |
| SD | AGGTTCAAAGGCAGGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12876A-B3 | VGB6 | C57BL/6NTac | |
| 12876A-D2 | VGB6 | C57BL/6NTac | |
| 12876A-B8 | VGB6 | C57BL/6NTac | |
| 12876A-G12 | VGB6 | C57BL/6NTac | |
