| Gene | 1600015I10Rik |
| Chromosome | 6 |
| Deletion Start | 48,930,089 |
| Deletion End | 48,933,561 |
| Deletion Size | 3,473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,930,532 | 48,930,553 | CAAAGCAGAGTACAGCCTCATC |
| TUP | 48,930,554 | 48,930,576 | TACCGTACGCTGACAGAGGCCAC |
| TUR | 48,930,592 | 48,930,613 | GTCAGTGGTGTCAAGGAAGAAC |
| TDF | 48,933,147 | 48,933,169 | TCCTAGTACCCACTGCCTATGTC |
| TDP | 48,933,174 | 48,933,197 | CTGTTGCCAACCACTGCCTAGACC |
| TDR | 48,933,206 | 48,933,230 | ACTCAGTAACTACGACTGCTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGACCCAGAAGGACAC |
| SD | AGAGGGTTTCTCTGCCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12878A-E2 | VGB6 | C57BL/6NTac | |
| 12878B-A6 | VGB6 | C57BL/6NTac | |
| 12878B-B5 | VGB6 | C57BL/6NTac | |
| 12878B-B6 | VGB6 | C57BL/6NTac | |
| 12878B-C2 | VGB6 | C57BL/6NTac | |
