| Gene | 2310047M10Rik |
| Chromosome | 11 |
| Deletion Start | 69,059,801 |
| Deletion End | 69,060,895 |
| Deletion Size | 1,095 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,059,932 | 69,059,951 | GGAAGAGGCTGAGAGCGTTG |
| TUP | 69,059,955 | 69,059,974 | TCTCGAGCCGTCATCCCCGA |
| TUR | 69,059,986 | 69,060,003 | ATGCTGCCGCTCCAAGTC |
| TDF | 69,060,658 | 69,060,675 | TGCGGTGCAAGAGCTGAC |
| TDP | 69,060,678 | 69,060,703 | CGTTGAGTGTGGGCTGTATCCAGACC |
| TDR | 69,060,729 | 69,060,750 | ATGCTCATGTCCACGGCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GTAATTCCGGACGACACCAC |
| SD | GCTTAGGGAGAAGGGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12882A-F3 | VGB6 | C57BL/6NTac | |
| 12882A-F6 | VGB6 | C57BL/6NTac | |
| 12882A-H5 | VGB6 | C57BL/6NTac | |
| 12882A-F5 | VGB6 | C57BL/6NTac | |
