| Gene | Fam123a |
| Chromosome | 14 |
| Deletion Start | 60,378,361 |
| Deletion End | 60,380,287 |
| Deletion Size | 1,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,378,519 | 60,378,536 | GCCGACGGGAATGGTGAG |
| TUP | 60,378,537 | 60,378,560 | AAGCAGGACCCACGACGGACTAGC |
| TUR | 60,378,574 | 60,378,592 | CCTTCTTGCTGCCCTCCAG |
| TDF | 60,379,738 | 60,379,758 | CCCATTGAGTCCCAGCAGAAG |
| TDP | 60,379,760 | 60,379,784 | AGGAGCCCAAACATCCGGAGAAGGA |
| TDR | 60,379,818 | 60,379,837 | GGGTGGTGGAGTCCCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCGATGAAGGCTCTTGCTAC |
| SD | TCTGCAAAGGCAAAGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12885A-A8 | VGB6 | C57BL/6NTac | |
| 12885A-C5 | VGB6 | C57BL/6NTac | |
| 12885A-E1 | VGB6 | C57BL/6NTac | |
| 12885A-E12 | VGB6 | C57BL/6NTac | |
| 12885A-G12 | VGB6 | C57BL/6NTac | |
| 12885A-H8 | VGB6 | C57BL/6NTac | |
