| Gene | 2700081O15Rik |
| Chromosome | 19 |
| Deletion Start | 7,421,443 |
| Deletion End | 7,423,141 |
| Deletion Size | 1,699 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,421,603 | 7,421,621 | ACCAGCCTGAGGCTCTGTC |
| TUP | 7,421,624 | 7,421,645 | AGCTCACCCAGCCCTCACCAGG |
| TUR | 7,421,724 | 7,421,745 | TGCAGCCCTCAACCAAATATCC |
| TDF | 7,422,587 | 7,422,606 | GGGACTGGGCCTGATTGATG |
| TDP | 7,422,608 | 7,422,630 | CACTCGCTCCAAGGCAGCCCTTT |
| TDR | 7,422,631 | 7,422,649 | GCGTCGCCTGGAGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTAGGCAGAGATCAGCCAC |
| SD | CCCTGCCTTCAACCCTAATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 143 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12886A-A8 | VGB6 | C57BL/6NTac | |
| 12886A-E1 | VGB6 | C57BL/6NTac | |
| 12886A-A12 | VGB6 | C57BL/6NTac | |
| 12886A-A2 | VGB6 | C57BL/6NTac | |
