| Gene | B3gnt1 |
| Chromosome | 19 |
| Deletion Start | 5,038,980 |
| Deletion End | 5,040,552 |
| Deletion Size | 1,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,039,090 | 5,039,108 | CCAGGAGGAGCAGGAACAG |
| TUP | 5,039,110 | 5,039,134 | ATTTCGAGTTCTTCCCGCCGTCTCC |
| TUR | 5,039,151 | 5,039,171 | GGTGCGGAGTTGAGACTTTAC |
| TDF | 5,039,809 | 5,039,828 | TCTATCAGGTGGGCGAAGTC |
| TDP | 5,039,829 | 5,039,853 | CGGCCCTTCTATTATGGGCTGTGCA |
| TDR | 5,039,876 | 5,039,894 | GTTGACCCAGCGGGAGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGGAAGGATTCAACCGGG |
| SD | TCAAGCTTCCCACCTCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12887A-D3 | VGB6 | C57BL/6NTac | |
| 12887A-G3 | VGB6 | C57BL/6NTac | |
| 12887A-G1 | VGB6 | C57BL/6NTac | |
| 12887A-D1 | VGB6 | C57BL/6NTac | |
